Protein Engineering, Expression and Production

Versatility at scale.

What matters most is not just speed but confidence. MindWalk’s US-based workflows streamline protein engineering, expression and production, delivering reproducible quality across conventional and complex recombinant proteins and antibody formats.

US-based protein expression and production

High-throughput and highly versatile protein formats

With more than two decades of experience in recombinant protein production, MindWalk delivers thoroughly characterized biomolecules with precision and consistency. Scalable workflows across discovery runs (100 µg–1 mg), medium-scale projects (10–50 mg), and gram-scale production are designed to support reliable performance from early experiments through advanced studies. Every program includes in silico analysis and optimization to support yield and quality.

From sequence to purified recombinant antibody:

3 weeks*


8-10 weeks for new custom proteins*
Timelines may vary depending on program complexity

Over 3 decades of experience

Demonstrated strategies, adaptable workflows, and a partnership approach to every challenge.

Quality + Scalability + Versatility

Protein Engineering

  • Engineered for complex architectures, including multi-specific and non-IgG formats, optimized for functionality and expression efficiency
  • Designed to enhance protein stability, solubility, and downstream yield
  • Built to transition into scalable production for consistent performance across applications

Protein Production

  • Rapid high-throughput to gram scale
  • From sequence to protein in as little as 3 weeks (For standard IgG)
  • Versatile formats

Cell Lines: + HEK293, CHO, E.coli

In Silico Integration: Codon Optimization

Recombinant antibody and protein formats

Protein engineering, expression, and production


MindWalk supports a diverse range of formats

  • Monospecific antibodies
  • Bispecific and multispecific antibodies
  • Fusion proteins and fusion constructs
  • Custom recombinant protein formats

Species

  • Human
  • Mouse
  • Rabbit
  • Llama
  • Other species and custom sequences

Scalable protein production workflows


Protein expression systems:

  • HEK293 transient expression
  • CHO transient and stable expression
  • E. coli expression

Protein customization and modification:

  • Protease Cleavage
  • Biochemical Modification
  • Biotinylation
  • Cross-linking
  • Custom protein modifications

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Expert services customized to your needs

Antibody engineering expression* is streamlined at MindWalk through workflows designed to reduce complexity while maintaining quality. Backed by a strong record of client retention, our services are offered as customized solutions that can be integrated into discovery programs. With dedicated scientists working alongside you, teams gain greater confidence as programs move forward.

*includes in silico biophysical profiling

Cutting-edge technology and versatile solutions

Our streamlined protein production workflows deliver high-quality, scalable results with consistency and workflow efficiency. Designed for versatility, they integrate with a wide range of programs to create customized solutions, from conventional formats to more complex requirements.

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Four ways to access MindWalk

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Partnership

We are proud to partner with the best in pharma, biotech and academia organizations. Join us in creating future of human health.